Age changes in the glycosaminoglycan composition of human bronchial cartilage
نویسندگان
چکیده
منابع مشابه
Age-related changes in the composition and structure of human articular-cartilage proteoglycans.
1. Analysis of the purified proteoglycans extracted from normal human articular cartilage with 4M-guanidinium chloride showed that there was an age-related increase in their content of protein and keratan sulphate. 2. The hydrodynamic size of the dissociated proteoglycans also decreased with advancing age, but there was little change in the proportion that could aggregate. 3. Results suggested ...
متن کاملChanges in the acid glycosaminoglycan content of the matrix of ageing human articular cartilage.
Early chemical studies of the glycosaminoglycans of cartilage matrix (Kuhn and Leppelmann, 1958; Kaplan and Meyer, 1959) indicated an increasing content of keratansulphate with maturation and ageing, particularly when considered in relation to the total quantity of mucopolysaccharide. Subsequently, the general distribution of chondroitin sulphate and keratansulphate within the matrix were demon...
متن کاملChanges with age in the glycosaminoglycans of human articular cartilage.
Human articular cartilage was obtained post mortem from the lateral femoral condyles of 30 subjects aged from under 1 to 70 years. Cryostat sections taken 0--100 micrometers and 900--100 micrometers deep to the cartilage surface were exhaustively extracted to recover the glycosaminoglycans (GAG). After fractionation by cellulose acetate electrophoresis and enzyme depolymerisation individual GAG...
متن کاملAge-related nanostructural and nanomechanical changes of individual human cartilage aggrecan monomers and their glycosaminoglycan side chains.
The nanostructure and nanomechanical properties of aggrecan monomers extracted and purified from human articular cartilage from donors of different ages (newborn, 29 and 38 year old) were directly visualized and quantified via atomic force microscopy (AFM)-based imaging and force spectroscopy. AFM imaging enabled direct comparison of full length monomers at different ages. The higher proportion...
متن کاملPrediction of glycosaminoglycan content in human cartilage by age, T1ρ and T2 MRI.
OBJECTIVE A relationship between T1ρ relaxation time and glycosaminoglycan (GAG) content has been demonstrated in chemically degraded bovine cartilage, but has not been demonstrated with quantitative biochemistry in human cartilage. A relationship has also been established between T2 relaxation time in cartilage and osteoarthritis (OA) severity. We hypothesized that T1ρ relaxation time would be...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1130030pb